ABSTRACT Objective: To study associations between polymorphisms in the angiotensin converting enzyme (ACE I/D), actinin 3 (ACTN3 R577X) and paraoxonase 1 (PON1 T(-107)C) genes and chronic diseases (diabetes and hypertension) in women. Materials and methods: Genomic DNA was extracted from saliva samples of 78 women between 18 and 59 years old used for genetic polymorphism screening. Biochemical data were collected from the medical records in Basic Health Units from Southern Brazil. Questionnaires about food consumption, physical activity level and socioeconomic status were applied. Results: The XX genotype of ACTN3 was associated with low HDL levels and high triglycerides, total cholesterol and glucose levels. Additionally, high triglycerides and LDL levels were observed in carriers of the TT genotype of PON1, and lower total cholesterol levels were associated to the CC genotype. As expected, women with diabetes/hypertense had increased body weight, BMI (p = 0.02), waist circumference (p = 0.01), body fat percentage, blood pressure (p = 0.02), cholesterol, triglycerides (p = 0.02), and blood glucose (p = 0.01), when compared to the control group. Conclusion: Both ACTN3 R577X and PON1 T(-107)C polymorphisms are associated with nutritional status and blood glucose and lipid levels in women with diabetes/hypertense. These results contribute to genetic knowledge about predisposition to obesity-related diseases.

RESUMO Objetivo Determinar a frequência dos alelos do Human leukocyte antigen e verificar a associação da presença desses alelos com sintomas e outras doenças relacionados à doença celíaca em portadores de doenças autoimunes da tireoide. Métodos Aplicou-se um questionário relacionado aos sintomas e doenças associados à doença celíaca. O ácido desoxirribonucleico genômico foi extraído por meio da coleta das células da mucosa bucal. Os alelos (DQA1*0501; DQB1*0201; DRB1*04) foram identificados por meio da reação em cadeia da polimerase. Resultados Participaram deste estudo 110 portadores de doenças autoimunes da tireoide. Observou-se que 66,4% dos indivíduos carregavam pelo menos um dos alelos estudados e que 58,2% dos indivíduos eram positivos para pelo menos um dos alelos DQ2 (DQA1*0501; DQB1*0201) e destes 18,2% foram positivos para ambos alelos do DQ2(DQA1*0501; DQB1*0201). Com relação ao DQ8 (DRB1*04), 21,8% da população estudada eram positivos para esse alelo e 3,6% eram positivos tanto para o DQ2 (DQA1*0501; DQB1*0201) quanto para o DQ8 (DRB1*04). Foi encontrada associação significativa da presença do alelo DRB1*04 com os sintomas gastrointestinais (p=0,02). Houve associação significativa do alelo DRB1*04 com diabetes mellitus tipo 1 (p=0,02). Conclusão O perfil genético mais fortemente associado à doença celíaca, tais como DQ2 (DQA1*0501; DQB1*0201) e DQ8 (DRB1*04) estavam presentes em torno de 20,0% da população estudada, estes são haplótipos de risco para doença celíaca e principalmente na presença de sintomas e doenças relacionadas à doença celíaca. Sendo assim, é importante realizar o rastreamento para investigar um possível diagnóstico para doença celíaca.

ABSTRACT Objective To determine the frequency of Human leukocyte antigen alleles and to verify the association of the presence of these alleles with symptoms and other diseases related to celiac disease in patients with autoimmune thyroid diseases. Methods A questionnaire on the symptoms and diseases associated with celiac disease was applied. Genomic deoxyribonucleic acid was extracted by collecting cells from the oral mucosa. The alleles (DQA1*0501; DQB1*0201; DRB1*04) were identified by means of the polymerase chain reaction. Results A total of 110 patients with autoimmune thyroid diseases participated in this study. It was observed that 66.4% of the individuals carried at least one of the alleles assessed and that 58.2% of the individuals were positive for at least one of the DQ2 alleles (DQA1*0501; DQB1*0201) and out of these 18.2% were positive for both DQ2 alleles (DQA1*0501; DQB1*0201). With regard to DQ8 (DRB1*04), 21.8% of the studied population was positive for this allele and 3.6% was positive for both DQ2 (DQA1*0501; DQB1*0201) and DQ8 (DRB1*04). A significant association was found between the presence of the DRB1*04 allele and gastrointestinal symptoms (p=0.02). A significant association of the DRB1*04 allele with type 1 diabetes mellitus (p=0.02) was observed. Conclusion The genetic profiles most commonly associated with celiac disease, such as DQ2 (DQA1*0501; DQB1*0201) and DQ8 (DRB1*04) were around 20.0% prevalent in the studied population. These are risk haplotypes for celiac disease especially when symptoms and diseases related to celiac disease are present. Therefore, it is important to screen patients to investigate a potential diagnosis for celiac disease.

Resumo Este estudo teve como objetivo verificar o perfil sociodemográfico, as condições de saúde e a relação com o consumo de produtos orgânicos de consumidores que frequentavam diferentes feiras agroecológicas no município de Pelotas – RS. Aplicou-se um questionário aos consumidores de produtos orgânicos durante as compras nas feiras agroecológicas. Observou-se que os consumidores com menor nível de escolaridade e renda passaram a frequentar mais as feiras agroecológicas consumindo mais alimentos orgânicos. Os consumidores estavam preocupados com saúde e alimentação, sendo pequena a parcela dos que apresentavam diagnóstico de doença, ocorrendo diferentes motivações que influenciavam a compra de alimentos orgânicos.

Abstract The scope of this study was to establish the socio-demographic profile, health status and the relationship with the consumption of organic produce among consumers who frequent different agroecological street markets in Pelotas in the state of Rio Grande do Sul. A questionnaire was given to the consumers of organic produce while shopping in the agroecological street markets. It was found that the consumers with lower education levels and lower income began to frequent the agroecological street markets more often and consume more organic foods. The consumers were concerned about their health and nutrition status with very few of them having a diagnosis of disease, there being different motivations influencing the purchase of organic food.

Resumo Este estudo teve como objetivo verificar o perfil sociodemográfico, as condições de saúde e a relação com o consumo de produtos orgânicos de consumidores que frequentavam diferentes feiras agroecológicas no município de Pelotas – RS. Aplicou-se um questionário aos consumidores de produtos orgânicos durante as compras nas feiras agroecológicas. Observou-se que os consumidores com menor nível de escolaridade e renda passaram a frequentar mais as feiras agroecológicas consumindo mais alimentos orgânicos. Os consumidores estavam preocupados com saúde e alimentação, sendo pequena a parcela dos que apresentavam diagnóstico de doença, ocorrendo diferentes motivações que influenciavam a compra de alimentos orgânicos.

Abstract The scope of this study was to establish the socio-demographic profile, health status and the relationship with the consumption of organic produce among consumers who frequent different agroecological street markets in Pelotas in the state of Rio Grande do Sul. A questionnaire was given to the consumers of organic produce while shopping in the agroecological street markets. It was found that the consumers with lower education levels and lower income began to frequent the agroecological street markets more often and consume more organic foods. The consumers were concerned about their health and nutrition status with very few of them having a diagnosis of disease, there being different motivations influencing the purchase of organic food.